Hotspot

trnL-UAA-trnT-UGU

LSC · 14418-15564 · 1147 bp

Back to Centrolobium

Pi 0.00437

MarkerSeek score 61.50

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 5
pi: 0.00437
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	TAGCGTCTACCAATTTCGCC	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.1	57.9	1205-1211 bp	1.0	78.9
trnL-UAA-trnT-UGU_p2	CCCATTGTTAATTCCGGGGT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	1290-1296 bp	1.0	78.2
trnL-UAA-trnT-UGU_p3	TAGCGTCTACCAATTTCGCC	GGAATCGAACCGATGACCAT	50.0	50.0	58.1	57.8	1237-1243 bp	1.0	78.0
trnL-UAA-trnT-UGU_p4	AGTCCGTAGCGTCTACCAAT	GCGATGCTCTAACCTCTGAG	50.0	55.0	58.2	57.9	1211-1217 bp	1.0	77.5
trnL-UAA-trnT-UGU_p5	CCCATTGTTAATTCCGGGGT	GGAATCGAACCGATGACCAT	50.0	50.0	57.8	57.8	1322-1328 bp	1.0	77.3