Hotspot

trnK-UUU-rbcL

LSC · 4393-5116 · 724 bp

Back to Centrolobium

Pi 0.00554

MarkerSeek score 62.20

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 4
pi: 0.00554
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	TACCGAGGGTATGGACGAAA	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.9	58.0	895-939 bp	1.0	79.2
trnK-UUU-rbcL_p2	TACCGAGGGTATGGACGAAA	GAGGCTAGCAAATGGAGTCC	50.0	55.0	57.9	58.0	910-954 bp	1.0	79.1
trnK-UUU-rbcL_p3	CTACCGAGGGTATGGACGAA	AGTCCTGAATTAGCTGCTGC	55.0	50.0	58.3	58.0	896-940 bp	1.0	77.8
trnK-UUU-rbcL_p4	CTACCGAGGGTATGGACGAA	GAGGCTAGCAAATGGAGTCC	55.0	55.0	58.3	58.0	911-955 bp	1.0	77.8
trnK-UUU-rbcL_p5	CGAGGGTATGGACGAAACAA	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.6	58.0	892-936 bp	1.0	77.3