Hotspot

rps15-ycf1

SSC · 122368-122790 · 423 bp

Back to Centaurea

Pi 0.00997

MarkerSeek score 48.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 423
variable_sites: 13
pi: 0.00997
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGTTTTCTTCTTGTTCTTGCA	AATAATGGGAGCCGCTTGAG	33.3	50.0	54.4	58.0	514-523 bp	1.0	46.9
rps15-ycf1_p2	TGTTTTCTTCTTGTTCTTGCAA	AATAATGGGAGCCGCTTGAG	31.8	50.0	55.2	58.0	514-523 bp	1.0	45.3
rps15-ycf1_p3	TTGTTTTCTTCTTGTTCTTGCA	AATAATGGGAGCCGCTTGAG	31.8	50.0	55.2	58.0	515-524 bp	1.0	45.3
rps15-ycf1_p4	TGTTTTCTTCTTGTTCTTGCA	CAATAATGGGAGCCGCTTGA	33.3	50.0	54.4	58.3	515-524 bp	1.0	44.5
rps15-ycf1_p5	CTTTGTTTTCTTCTTGTTCTTGCA	AATAATGGGAGCCGCTTGAG	33.3	50.0	57.1	58.0	517-526 bp	1.0	43.7