Hotspot

ndhG-ndhI

SSC · 117711-118083 · 373 bp

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Pi 0.0153

MarkerSeek score 44.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 373
variable_sites: 17
pi: 0.0153
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCCGTACCATGACGTATCTG	TTGATCACATCCGAGCGTTT	55.0	45.0	58.2	57.9	1049-1102 bp	1.0	82.9
ndhG-ndhI_p2	AATCCCAACGAAAAGGCAGA	GATGGACTTTACCGAGGCTG	45.0	55.0	57.7	58.1	1140-1193 bp	0.909091	79.0
ndhG-ndhI_p3	ATCCCAACGAAAAGGCAGAA	GATGGACTTTACCGAGGCTG	45.0	55.0	57.7	58.1	1139-1192 bp	0.909091	79.0
ndhG-ndhI_p4	TCCCAACGAAAAGGCAGAAT	GATGGACTTTACCGAGGCTG	45.0	55.0	57.7	58.1	1138-1191 bp	0.909091	79.0
ndhG-ndhI_p5	GCCGTACCATGACGTATCTG	GATGGACTTTACCGAGGCTG	55.0	55.0	58.2	58.1	1384-1437 bp	0.909091	78.6