Hotspot

rpl32-trnL-UAG

SSC · 112307-113120 · 814 bp

Back to Centaurea

Pi 0.00998
MarkerSeek score 41.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
814
variable_sites
26
pi
0.00998
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGAAAGGATATTGGGCGG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.6 58.0 947-1018 bp 1.0 79.2
rpl32-trnL-UAG_p2 AAGGAAAGGATATTGGGCGG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.6 58.0 948-1019 bp 1.0 79.2
rpl32-trnL-UAG_p3 CGGCGTTAAAAGCTTTGTCA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.6 58.0 930-1001 bp 1.0 79.1
rpl32-trnL-UAG_p4 CGGCGTTAAAAGCTTTGTCA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.6 58.0 931-1002 bp 1.0 79.0
rpl32-trnL-UAG_p5 AAGGAAAGGATATTGGGCGG TGATCCACAAAGAAGAGCCG 50.0 50.0 57.6 57.9 1604-1675 bp 1.0 78.6