Hotspot

trnS-GGA-rps4

LSC · 44729-45038 · 310 bp

Back to Centaurea

Pi 0.00821

MarkerSeek score 39.20

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 310
variable_sites: 8
pi: 0.00821
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TCGAATCCCTCTCTTTCCGT	AGTAAATGGGTCGGTTTGAA	50.0	40.0	58.2	54.9	394-396 bp	1.0	55.8
trnS-GGA-rps4_p2	TCGAATCCCTCTCTTTCCGT	AGTAAATGGGTCGGTTTGAAA	50.0	38.1	58.2	55.7	394-396 bp	1.0	54.4
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	AGTAAATGGGTCGGTTTGAA	55.0	40.0	58.4	54.9	467-469 bp	1.0	53.7
trnS-GGA-rps4_p4	TAGGCTTTTGCTTACCGAGG	AGTAAATGGGTCGGTTTGAA	50.0	40.0	57.6	54.9	416-418 bp	1.0	53.7
trnS-GGA-rps4_p5	TTTTGCTTACCGAGGGTTCG	AGTAAATGGGTCGGTTTGAA	50.0	40.0	58.5	54.9	411-413 bp	1.0	53.0