Hotspot

atpI-atpH

LSC · 24784-25926 · 1143 bp

Back to Centaurea

Pi 0.00439

MarkerSeek score 50.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 17
pi: 0.00439
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	50.0	45.0	58.3	58.0	1265-1279 bp	1.0	79.9
atpI-atpH_p2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	50.0	50.0	58.3	57.9	1321-1335 bp	1.0	79.3
atpI-atpH_p3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	50.0	45.0	58.3	57.9	1281-1295 bp	1.0	79.0
atpI-atpH_p4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	50.0	45.0	58.3	58.2	1266-1280 bp	1.0	78.8
atpI-atpH_p5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	50.0	55.0	58.3	58.2	1325-1339 bp	1.0	78.6