Hotspot

petN-psbM

LSC · 9995-10499 · 505 bp

Back to Centaurea

Pi 0.0103

MarkerSeek score 44.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 505
variable_sites: 19
pi: 0.0103
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGGAAGAAGTGGGCTTTAG	ACGTAAAAACAGTCAGTCAA	55.0	35.0	57.9	53.0	554-560 bp	1.0	42.7
petN-psbM_p2	GGGGAAGAAGTGGGCTTTAG	ACGTAAAAACAGTCAGTCAAA	55.0	33.3	57.9	53.9	554-560 bp	1.0	42.7
petN-psbM_p3	GGGGAAGAAGTGGGCTTTAG	ACGTAAAAACAGTCAGTCAAAATGA	55.0	32.0	57.9	57.9	554-560 bp	1.0	42.7
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACAGTCAGTCAA	50.0	35.0	58.2	53.0	617-623 bp	1.0	42.5
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACAGTCAGTCAAA	50.0	33.3	58.2	53.9	617-623 bp	1.0	42.5