Hotspot

trnK-UUU-rps16

LSC · 4341-5115 · 775 bp

Back to Centaurea

Pi 0.00807
MarkerSeek score 45.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
775
variable_sites
21
pi
0.00807
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 973-1004 bp 1.0 78.7
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 972-1003 bp 1.0 78.7
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 912-943 bp 1.0 78.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 911-942 bp 1.0 78.2
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC ACTTTGCCCTAATCAAGCGA 55.0 45.0 57.9 57.5 901-932 bp 1.0 77.4