Hotspot

rpoA

LSC · 82228-83223 · 996 bp

Back to Cenostigma

Pi 0.00201

MarkerSeek score 45.40

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 996
variable_sites: 2
pi: 0.00201
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AAAACCCATTTCGTCGTCCA	TGGATGTAGGCCCCCTAAAA	45.0	50.0	58.0	58.0	1428 bp	1.0	80.2
rpoA_p2	TCCAAAACCCATTTCGTCGT	TGGATGTAGGCCCCCTAAAA	45.0	50.0	58.0	58.0	1431 bp	1.0	80.2
rpoA_p3	AAAACCCATTTCGTCGTCCA	GTACGGGGTCAGGTGATTTC	45.0	55.0	58.0	58.0	1676 bp	1.0	80.1
rpoA_p4	TCCAAAACCCATTTCGTCGT	GTACGGGGTCAGGTGATTTC	45.0	55.0	58.0	58.0	1679 bp	1.0	80.1
rpoA_p5	AAAACCCATTTCGTCGTCCA	TCACGTAAGAATGCGCGTAA	45.0	45.0	58.0	58.0	1760 bp	1.0	80.1