Hotspot

rpl32-trnL-UAG

SSC · 118450-118840 · 391 bp

Back to Cenostigma

Pi 0.0256

MarkerSeek score 66.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 391
variable_sites: 10
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCATTGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	520-524 bp	1.0	81.5
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCATTGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	521-525 bp	1.0	81.5
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCATTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	521-525 bp	1.0	81.4
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCATTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	522-526 bp	1.0	81.4
rpl32-trnL-UAG_p5	GAGAAGGGATATTGGGCAGC	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	525-529 bp	1.0	80.7