Hotspot

ndhF-rpl32

SSC · 117371-118281 · 911 bp

Back to Cenostigma

Pi 0.00774
MarkerSeek score 63.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
911
variable_sites
7
pi
0.00774
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGTGGGAAAAAGGAGAAGT TCAATGCTGCCCAATATCCC 50.0 50.0 57.7 58.0 1086-1093 bp 1.0 78.8
ndhF-rpl32_p2 CCGTGGGAAAAAGGAGAAGT CAATGCTGCCCAATATCCCT 50.0 50.0 57.7 58.0 1085-1092 bp 1.0 78.8
ndhF-rpl32_p3 TTTGTTGCCGTGGGAAAAAG TCAATGCTGCCCAATATCCC 45.0 50.0 57.7 58.0 1093-1100 bp 1.0 78.6
ndhF-rpl32_p4 TTTGTTGCCGTGGGAAAAAG CAATGCTGCCCAATATCCCT 45.0 50.0 57.7 58.0 1092-1099 bp 1.0 78.6
ndhF-rpl32_p5 CCGTGGGAAAAAGGAGAAGT GCTGCCCAATATCCCTTCTC 50.0 55.0 57.7 58.1 1081-1088 bp 1.0 78.1