Hotspot

rps7-rps12

IRb · 102757-102809 · 53 bp

Back to Cenostigma

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	ATTGGATCGGATTTTGCGGT	CTGTGGAAAGCCGTATTCGA	45.0	50.0	58.2	58.0	208 bp	1.0	78.4
rps7-rps12_p2	ATTGGATCGGATTTTGCGGT	TCTGTGGAAAGCCGTATTCG	45.0	50.0	58.2	58.0	209 bp	1.0	78.4
rps7-rps12_p3	TGGATCGGATTTTGCGGTTT	CTGTGGAAAGCCGTATTCGA	45.0	50.0	58.5	58.0	206 bp	1.0	76.7
rps7-rps12_p4	TGGATCGGATTTTGCGGTTT	TCTGTGGAAAGCCGTATTCG	45.0	50.0	58.5	58.0	207 bp	1.0	76.7
rps7-rps12_p5	TTGGATCGGATTTTGCGGTT	CTGTGGAAAGCCGTATTCGA	45.0	50.0	58.5	58.0	207 bp	1.0	76.7