Hotspot

rps11-rpl36

LSC · 83712-84056 · 345 bp

Back to Cenostigma

Pi 0.0339

MarkerSeek score 73.00

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 345
variable_sites: 10
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TACGCGCATTCTTACGTGAA	CGTAGACGCGGACGAATTAT	45.0	50.0	58.0	58.0	428-478 bp	1.0	85.4
rps11-rpl36_p2	TTACGCGCATTCTTACGTGA	CGTAGACGCGGACGAATTAT	45.0	50.0	58.0	58.0	429-479 bp	1.0	85.4
rps11-rpl36_p3	CCCTTTGGTATTTTACGCGC	CGTAGACGCGGACGAATTAT	50.0	50.0	57.8	58.0	441-491 bp	1.0	84.0
rps11-rpl36_p4	TTTACGCGCATTCTTACGTG	CGTAGACGCGGACGAATTAT	45.0	50.0	57.2	58.0	430-480 bp	1.0	79.3
rps11-rpl36_p5	TACGCGCATTCTTACGTGAA	AAGAGCCTCCGTTCGTAAAA	45.0	45.0	58.0	57.2	471-521 bp	1.0	78.8