Hotspot

psbK-psbI

LSC · 8181-8584 · 404 bp

Back to Cenostigma

Pi 0.0075

MarkerSeek score 52.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 3
pi: 0.0075
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.1	57.3	543-547 bp	1.0	75.3
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCCGGATTAC	50.0	50.0	57.9	57.3	538-542 bp	1.0	75.3
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	GGATTACGTCCTGGATCGTT	45.0	50.0	58.1	57.1	530-534 bp	1.0	73.5
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	GGATTACGTCCTGGATCGTT	50.0	50.0	57.9	57.1	525-529 bp	1.0	73.5
psbK-psbI_p5	TGGCAAGCTGCTGTAAGTTT	TTCTTCACGTCCCGGATTAC	45.0	50.0	58.3	57.3	535-539 bp	1.0	73.3