Hotspot

trnQ-UUG-psbK

LSC · 7452-7994 · 543 bp

Back to Cenostigma

Pi 0.0173

MarkerSeek score 62.70

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 543
variable_sites: 9
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	TACTCTGGGACGGAAGGATT	ACTTGAATAAAGGGCGGAGT	50.0	45.0	57.5	56.8	676-683 bp	1.0	70.1
trnQ-UUG-psbK_p2	GACCAAAACCCGTTGCCTTA	ACTTGAATAAAGGGCGGAGT	50.0	45.0	58.7	56.8	638-645 bp	1.0	69.3
trnQ-UUG-psbK_p3	ATACTCTGGGACGGAAGGAT	ACTTGAATAAAGGGCGGAGT	50.0	45.0	57.2	56.8	677-684 bp	1.0	68.3
trnQ-UUG-psbK_p4	ACTCTGGGACGGAAGGATTC	ACTTGAATAAAGGGCGGAGT	55.0	45.0	58.8	56.8	675-682 bp	1.0	68.1
trnQ-UUG-psbK_p5	TACTCTGGGACGGAAGGATT	ACTTGAATAAAGGGCGGAGTT	50.0	42.9	57.5	57.6	676-683 bp	1.0	68.0