Hotspot

trnK-UUU-rps16

LSC · 4374-5598 · 1225 bp

Back to Cenostigma

Pi 0.00911

MarkerSeek score 65.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1225
variable_sites: 11
pi: 0.00911
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	AAAAAGGCGCTCAACCTACA	55.0	45.0	57.9	58.0	1413-1417 bp	1.0	80.8
trnK-UUU-rps16_p2	CCGATGGTATGGACGAATCC	CCTTGAAAAAGGCGCTCAAC	55.0	50.0	57.9	58.2	1418-1422 bp	1.0	79.2
trnK-UUU-rps16_p3	CCGATGGTATGGACGAATCC	CTTGAAAAAGGCGCTCAACC	55.0	50.0	57.9	58.2	1417-1421 bp	1.0	79.2
trnK-UUU-rps16_p4	CCGATGGTATGGACGAATCC	TGAAAAAGGCGCTCAACCTA	55.0	45.0	57.9	57.7	1415-1419 bp	1.0	78.9
trnK-UUU-rps16_p5	CCGATGGTATGGACGAATCC	AGGCCGGTGTTTTTATGGAA	55.0	45.0	57.9	57.7	1368-1372 bp	1.0	78.7