Hotspot

ndhI-ndhA

SSC · 112700-112790 · 91 bp

Back to Cenchrus

Pi 0.0275

MarkerSeek score 61.50

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 91
variable_sites: 5
pi: 0.0275
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TGACCAATGTATCTTGCGGC	TGGACTTTACCCAGGATGAGA	50.0	47.6	58.6	57.8	279-298 bp	1.0	68.6
ndhI-ndhA_p2	TGACCAATGTATCTTGCGGC	ACCCAGGATGAGAATGGATCA	50.0	47.6	58.6	58.2	271-290 bp	1.0	68.5
ndhI-ndhA_p3	CGCGTATTGTTTGTTGACCA	TGGACTTTACCCAGGATGAGA	45.0	47.6	57.3	57.8	260-279 bp	1.0	68.4
ndhI-ndhA_p4	GCGCGTATTGTTTGTTGACC	TGGACTTTACCCAGGATGAGA	50.0	47.6	58.7	57.8	261-280 bp	1.0	68.3
ndhI-ndhA_p5	GGCGCGTATTGTTTGTTGAC	TGGACTTTACCCAGGATGAGA	50.0	47.6	58.7	57.8	262-281 bp	1.0	68.3