Hotspot

rps3-rpl22

LSC · 80346-80403 · 58 bp

Back to Cenchrus

Pi 0.0295

MarkerSeek score 64.10

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 5
pi: 0.0295
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	ACCAAGTCTGAAACCGAGTG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.8	57.7	193-233 bp	0.916667	74.8
rps3-rpl22_p2	GTGCGAACCAAAAGGAATGG	CGAGCTCGAGGACGTAGTTA	50.0	55.0	57.9	58.7	215-255 bp	0.916667	72.9
rps3-rpl22_p3	CCAAGTCTGAAACCGAGTGG	AAATTCAGACCTCGAGCTCG	55.0	50.0	58.5	57.7	192-232 bp	0.916667	72.8
rps3-rpl22_p4	CAAGTCTGAAACCGAGTGGA	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.5	57.7	191-231 bp	0.916667	72.5
rps3-rpl22_p5	GTGCGAACCAAAAGGAATGG	AAATTCAGACCTCGAGCTCG	50.0	50.0	57.9	57.7	227-267 bp	0.833333	72.2