Hotspot

petA-psbJ

LSC · 61039-61919 · 881 bp

Back to Cenchrus

Pi 0.0179

MarkerSeek score 77.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 881
variable_sites: 57
pi: 0.0179
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	ACGGTTCGTATTCTGGATTGG	50.0	47.6	57.9	58.1	962-1045 bp	1.0	79.5
petA-psbJ_p2	GCATCCAAGGCCTTTTGTTC	ACGGTTCGTATTCTGGATTGG	50.0	47.6	57.9	58.1	970-1053 bp	1.0	79.2
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	GGTTCGTATTCTGGATTGGGT	50.0	47.6	57.9	57.7	960-1043 bp	1.0	78.0
petA-psbJ_p4	GCATCCAAGGCCTTTTGTTC	GGTTCGTATTCTGGATTGGGT	50.0	47.6	57.9	57.7	968-1051 bp	1.0	77.8
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CGGTTCGTATTCTGGATTGG	50.0	50.0	57.9	56.3	961-1044 bp	1.0	74.8