Hotspot

ndhC-trnM-CAT

LSC · 50881-52572 · 1692 bp

Back to Cenchrus

Pi 0.0114

MarkerSeek score 52.80

Primer available yes

Label ndhC-trnM-CAT

Feature Metadata

label_name: ndhC-trnM-CAT
feature_type: igs
region: LSC
length_bp: 1692
variable_sites: 54
pi: 0.0114
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnM-CAT_p1	TCATATTCGTGAAGCAGAAACA	CCCGCCGTATGAAAGCAATA	36.4	50.0	56.1	58.4	1585-1833 bp	1.0	52.2
ndhC-trnM-CAT_p2	TCATATTCGTGAAGCAGAAACA	CCGCCGTATGAAAGCAATAC	36.4	50.0	56.1	57.3	1584-1832 bp	1.0	50.2
ndhC-trnM-CAT_p3	TCATATTCGTGAAGCAGAAACA	TTTGAACCAATGACTCCCGC	36.4	50.0	56.1	58.8	1600-1848 bp	1.0	49.4
ndhC-trnM-CAT_p4	CATATTCGTGAAGCAGAAACA	CCCGCCGTATGAAAGCAATA	38.1	50.0	54.6	58.4	1584-1832 bp	1.0	48.2
ndhC-trnM-CAT_p5	TCATATTCGTGAAGCAGAAACAT	CCCGCCGTATGAAAGCAATA	34.8	50.0	56.6	58.4	1585-1833 bp	1.0	48.2