Hotspot

petN-trnC-GCA

LSC · 18063-18919 · 857 bp

Back to Cenchrus

Pi 0.0151

MarkerSeek score 65.10

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 857
variable_sites: 46
pi: 0.0151
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	CTGCAAATCCTTTACCCCCA	50.0	50.0	58.2	57.8	896-1025 bp	1.0	84.8
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	TGCAAATCCTTTACCCCCAG	50.0	50.0	58.2	57.8	895-1024 bp	1.0	84.8
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	ACTGCAAATCCTTTACCCCC	50.0	50.0	58.2	57.8	897-1026 bp	1.0	84.8
petN-trnC-GCA_p4	AGCCCAAGCGAGACTTACTA	GCAAATCCTTTACCCCCAGT	50.0	50.0	58.2	57.8	894-1023 bp	1.0	84.8
petN-trnC-GCA_p5	CCACTCCTCCCCCATACTAC	TGCAAATCCTTTACCCCCAG	60.0	50.0	58.3	57.8	951-1080 bp	1.0	83.6