Hotspot

trnD-GTC-psbM

LSC · 16104-17128 · 1025 bp

Back to Cenchrus

Pi 0.0128

MarkerSeek score 61.80

Primer available yes

Label trnD-GTC-psbM

Feature Metadata

label_name: trnD-GTC-psbM
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 55
pi: 0.0128
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GTC-psbM_p1	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTT	50.0	40.0	58.5	56.7	1164-1250 bp	1.0	73.5
trnD-GTC-psbM_p2	TTCAATTGGTCAGAGCACCG	AATCATTTTGGCTGGCTGTT	50.0	40.0	58.5	56.5	1166-1252 bp	1.0	71.6
trnD-GTC-psbM_p3	TTCAATTGGTCAGAGCACCG	ATCATTTTGGCTGGCTGTTT	50.0	40.0	58.5	56.5	1165-1251 bp	1.0	71.6
trnD-GTC-psbM_p4	TTCAATTGGTCAGAGCACCG	TCATTTTGGCTGGCTGTTTTT	50.0	38.1	58.5	57.4	1164-1250 bp	1.0	71.4
trnD-GTC-psbM_p5	TTCAATTGGTCAGAGCACCG	TTTGGCTGGCTGTTTTTACA	50.0	40.0	58.5	56.3	1160-1246 bp	1.0	70.7