Hotspot

trnfM-CAT-trnT-GGT

LSC · 12934-14861 · 1928 bp

Back to Cenchrus

Pi 0.0129

MarkerSeek score 60.90

Primer available yes

Label trnfM-CAT-trnT-GGT

Feature Metadata

label_name: trnfM-CAT-trnT-GGT
feature_type: igs
region: LSC
length_bp: 1928
variable_sites: 85
pi: 0.0129
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAT-trnT-GGT_p1	CCTCAAGGTTATGAGCCTCG	ACCACTAAGTGAAAAGCCCT	55.0	45.0	57.8	56.7	2061-2748 bp	1.0	74.0
trnfM-CAT-trnT-GGT_p2	CCTCAAGGTTATGAGCCTCG	TGAACCGATGACTTATGCCT	55.0	45.0	57.8	56.6	2031-2718 bp	1.0	73.8
trnfM-CAT-trnT-GGT_p3	CCTCGTGAGCTACCAAACTG	ACCACTAAGTGAAAAGCCCT	55.0	45.0	58.3	56.7	2046-2733 bp	1.0	73.6
trnfM-CAT-trnT-GGT_p4	CCTCGTGAGCTACCAAACTG	TGAACCGATGACTTATGCCT	55.0	45.0	58.3	56.6	2016-2703 bp	1.0	73.4
trnfM-CAT-trnT-GGT_p5	CCTCAAGGTTATGAGCCTCG	CCGATGACTTATGCCTTACCA	55.0	47.6	57.8	57.5	2027-2714 bp	1.0	73.0