Hotspot

trnG-GCC-trnfM-CAT

LSC · 12512-12859 · 348 bp

Back to Cenchrus

Pi 0.0214

MarkerSeek score 52.10

Primer available yes

Label trnG-GCC-trnfM-CAT

Feature Metadata

label_name: trnG-GCC-trnfM-CAT
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 18
pi: 0.0214
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAT_p1	GGCGTAGTTCAATGTAGCGA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	402-542 bp	1.0	84.3
trnG-GCC-trnfM-CAT_p2	GGCGTAGTTCAATGTAGCGA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.1	58.3	417-557 bp	1.0	83.8
trnG-GCC-trnfM-CAT_p3	GGCGTAGTTCAATGTAGCGA	CTACAGAGCGGAGTAGAGCA	50.0	55.0	58.1	58.3	435-575 bp	1.0	83.4
trnG-GCC-trnfM-CAT_p4	GGCGTAGTTCAATGTAGCGA	TACAGAGCGGAGTAGAGCAG	50.0	55.0	58.1	58.3	434-574 bp	1.0	83.4
trnG-GCC-trnfM-CAT_p5	GGCGTAGTTCAATGTAGCGA	GAGCGGAGTAGAGCAGTTTG	50.0	55.0	58.1	58.4	430-570 bp	1.0	83.1