Hotspot

psbZ-trnG-GCC

LSC · 12136-12440 · 305 bp

Back to Cenchrus

Pi 0.0213

MarkerSeek score 65.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 305
variable_sites: 26
pi: 0.0213
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	TCGAACCCGTATCTTCTCCT	50.0	50.0	57.8	57.6	443-471 bp	1.0	83.7
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	TCGAACCCGTATCTTCTCCT	55.0	50.0	58.3	57.6	441-469 bp	1.0	83.3
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	TTACCACTGAACTACGCTCG	50.0	50.0	57.8	57.4	408-436 bp	1.0	82.6
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	TTACCACTGAACTACGCTCG	55.0	50.0	58.3	57.4	406-434 bp	1.0	82.2
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	CCGTATCTTCTCCTTGGCAA	50.0	50.0	57.8	57.3	437-465 bp	1.0	81.7