Hotspot

ycf1

SSC · 127175-132931 · 5757 bp

Pi 0.00421

MarkerSeek score 38.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5757
variable_sites: 86
pi: 0.00421
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATTCAGCAACCAGTCTGG	ACTCGCAAAAATGAACCCCA	50.0	45.0	57.8	58.3	1038 bp	0.9	73.5
ycf1_p2	ATATTGATCCAGGCCCCCAT	ACTCGCAAAAATGAACCCCA	50.0	45.0	58.2	58.3	1347-1356 bp	0.9	72.9
ycf1_p3	ATTGATCCAGGCCCCCATAT	ACTCGCAAAAATGAACCCCA	50.0	45.0	58.2	58.3	1345-1354 bp	0.9	72.9
ycf1_p4	GATCCAGGCCCCCATATCTA	ACTCGCAAAAATGAACCCCA	55.0	45.0	57.7	58.3	1342-1351 bp	0.9	72.6
ycf1_p5	TATTGATCCAGGCCCCCATA	ACTCGCAAAAATGAACCCCA	50.0	45.0	57.6	58.3	1346-1355 bp	0.9	71.1