Hotspot

psbC

LSC · 35283-36704 · 1422 bp

Pi 0.00178

MarkerSeek score 38.40

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 7
pi: 0.00178
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCTCAGGAAATCCGTGCAG	CAAGCATTGGGTCTCTCGTC	50.0	55.0	57.9	58.6	1552 bp	1.0	74.5
psbC_p2	TTCTCAGGAAATCCGTGCAG	TTCAATATCACCCGACCCAC	50.0	50.0	57.9	57.3	1613 bp	1.0	74.0
psbC_p3	TTCTCAGGAAATCCGTGCAG	CCGACCCACCCAACCTAATA	50.0	55.0	57.9	58.8	1602 bp	1.0	73.3
psbC_p4	GCAGCGGAAGATCCTGAATT	CAAGCATTGGGTCTCTCGTC	50.0	55.0	58.3	58.6	1536 bp	1.0	72.7
psbC_p5	AGGGTATTCGTGCTTGGATG	CAAGCATTGGGTCTCTCGTC	50.0	55.0	57.7	58.6	1481 bp	1.0	72.6