Hotspot

psaC-ndhE

SSC · 120566-120837 · 272 bp

Back to Celtis

Pi 0.00743
MarkerSeek score 52.40
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
272
variable_sites
7
pi
0.00743
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 CTTAGCTTTACACCCGTCCC CTGAAGCAGCTATTGGACCA 55.0 50.0 58.0 57.6 469-475 bp 1.0 78.8
psaC-ndhE_p2 CTTAGCTTTACACCCGTCCC TGAAGCAGCTATTGGACCAG 55.0 50.0 58.0 57.6 468-474 bp 1.0 78.8
psaC-ndhE_p3 TTACACCCGTCCCAAGGTAT CTGAAGCAGCTATTGGACCA 50.0 50.0 58.0 57.6 462-468 bp 1.0 78.6
psaC-ndhE_p4 TTACACCCGTCCCAAGGTAT TGAAGCAGCTATTGGACCAG 50.0 50.0 58.0 57.6 461-467 bp 1.0 78.6
psaC-ndhE_p5 TGCTTAGCTTTACACCCGTC TGAAGCAGCTATTGGACCAG 50.0 50.0 57.9 57.6 470-476 bp 1.0 78.2