Hotspot

rpl32-trnL-UAG

SSC · 116258-117200 · 943 bp

Back to Celtis

Pi 0.0123
MarkerSeek score 61.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
943
variable_sites
42
pi
0.0123
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1049-1115 bp 1.0 83.4
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1050-1116 bp 1.0 83.3
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1059-1125 bp 1.0 82.4
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.8 57.8 1058-1124 bp 1.0 81.8
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 1057-1123 bp 1.0 81.8