Hotspot

ndhF-rpl32

SSC · 114905-116083 · 1179 bp

Back to Celtis

Pi 0.00912
MarkerSeek score 61.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1179
variable_sites
36
pi
0.00912
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTTGTTGCCGCTGGAAAAAG AAAAGCTTTCAATGCTGCCC 45.0 45.0 58.1 57.8 1376-1400 bp 1.0 83.3
ndhF-rpl32_p2 TGCCGCTGGAAAAAGTAGAA AAAAGCTTTCAATGCTGCCC 45.0 45.0 57.7 57.8 1371-1395 bp 1.0 81.7
ndhF-rpl32_p3 TTGCCGCTGGAAAAAGTAGA AAAAGCTTTCAATGCTGCCC 45.0 45.0 57.7 57.8 1372-1396 bp 1.0 81.7
ndhF-rpl32_p4 TGTTGCCGCTGGAAAAAGTA AAAAGCTTTCAATGCTGCCC 45.0 45.0 58.3 57.8 1374-1398 bp 1.0 81.3
ndhF-rpl32_p5 GTTGCCGCTGGAAAAAGTAG AAAAGCTTTCAATGCTGCCC 50.0 45.0 57.7 57.8 1373-1397 bp 1.0 81.2