Hotspot

rps8-rpl14

LSC · 83030-83238 · 209 bp

Back to Celtis

Pi 0.0116
MarkerSeek score 52.50
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
209
variable_sites
7
pi
0.0116
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 TGTCAGCATTTCGTATAGAGGT GAACTCGAATTTTTGGCGCA 40.9 45.0 57.0 58.0 347-351 bp 1.0 57.7
rps8-rpl14_p2 TGTCAGCATTTCGTATAGAGGT CGAATTTTTGGCGCAATTGC 40.9 45.0 57.0 58.1 342-346 bp 1.0 57.0
rps8-rpl14_p3 TGTCAGCATTTCGTATAGAGGT GCAATTGCCCGGGAATTAAG 40.9 50.0 57.0 57.8 330-334 bp 1.0 56.4
rps8-rpl14_p4 ACGGTATTCGAACTGTTCTT GAACTCGAATTTTTGGCGCA 40.0 45.0 54.7 58.0 377-381 bp 1.0 55.5
rps8-rpl14_p5 AGCAATCGTGTCTTTACTCA GAACTCGAATTTTTGGCGCA 40.0 45.0 54.7 58.0 316-320 bp 1.0 55.3