Hotspot

psbZ-trnG-GCC

LSC · 37472-37942 · 471 bp

Back to Celtis

Pi 0.00769
MarkerSeek score 59.10
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
471
variable_sites
11
pi
0.00769
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTACTGATGGTTGGTTGGGT AATCGAACCCGCATCTTCTC 50.0 50.0 57.1 58.1 594-665 bp 1.0 77.9
psbZ-trnG-GCC_p2 CTACTGATGGTTGGTTGGGT GAATCGAACCCGCATCTTCT 50.0 50.0 57.1 58.1 595-666 bp 1.0 77.9
psbZ-trnG-GCC_p3 TCTACTGATGGTTGGTTGGG AATCGAACCCGCATCTTCTC 50.0 50.0 56.8 58.1 595-666 bp 1.0 75.5
psbZ-trnG-GCC_p4 TCTACTGATGGTTGGTTGGG GAATCGAACCCGCATCTTCT 50.0 50.0 56.8 58.1 596-667 bp 1.0 75.5
psbZ-trnG-GCC_p5 ACTGATGGTTGGTTGGGTAA AATCGAACCCGCATCTTCTC 45.0 50.0 56.6 58.1 592-663 bp 1.0 74.0