Hotspot

trnT-GGU-psbD

LSC · 33063-34273 · 1211 bp

Back to Celtis

Pi 0.0101
MarkerSeek score 57.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1211
variable_sites
47
pi
0.0101
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG AAGCAATAGACCGGACCAAC 55.0 50.0 58.1 57.9 1383-1562 bp 1.0 81.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA AAGCAATAGACCGGACCAAC 50.0 50.0 58.1 57.9 1386-1565 bp 1.0 81.4
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1361-1540 bp 1.0 80.5
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG GACCGGACCAACCTACAAAA 55.0 50.0 58.1 57.7 1375-1554 bp 1.0 80.4
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1364-1543 bp 1.0 80.4