Hotspot

trnE-UUC-trnT-GGU

LSC · 32316-32990 · 675 bp

Back to Celtis

Pi 0.0113
MarkerSeek score 56.30
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
675
variable_sites
24
pi
0.0113
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AGAGATGTCCTGAACCGCTA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 750-769 bp 1.0 83.2
trnE-UUC-trnT-GGU_p2 AGATGTCCTGAACCGCTAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 748-767 bp 1.0 83.2
trnE-UUC-trnT-GGU_p3 AGAGATGTCCTGAACCGCTA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 729-748 bp 1.0 82.9
trnE-UUC-trnT-GGU_p4 AGATGTCCTGAACCGCTAGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.9 58.1 727-746 bp 1.0 82.9
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 804-823 bp 1.0 82.8