Hotspot

trnC-GCA-petN

LSC · 28657-29646 · 990 bp

Back to Celtis

Pi 0.00355
MarkerSeek score 52.70
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
990
variable_sites
16
pi
0.00355
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG TCTAGAGTCCACTTCGTCCC 50.0 55.0 58.1 57.9 1098-1131 bp 1.0 80.4
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 1034-1067 bp 1.0 80.3
trnC-GCA-petN_p3 GTATCATTTTGGCGGCATGG TCTAGAGTCCACTTCGTCCC 50.0 55.0 57.9 57.9 1150-1183 bp 1.0 79.9
trnC-GCA-petN_p4 GTATCATTTTGGCGGCATGG AGCCCAAGCGAGACTTACTA 50.0 50.0 57.9 58.2 1086-1119 bp 1.0 79.8
trnC-GCA-petN_p5 TTTCCCCAGTTCAAATCCGG CGTCCCCATACTACGAGTGA 50.0 55.0 58.1 58.3 1084-1117 bp 1.0 78.8