Hotspot

trnK-UUU-rps16

LSC · 4381-5144 · 764 bp

Back to Celtis

Pi 0.00813
MarkerSeek score 59.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
764
variable_sites
19
pi
0.00813
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGCACTTAAAAGCCGAGTA TCAAAGAAGGCAGGGGTTTT 50.0 45.0 58.3 57.5 841-867 bp 1.0 79.6
trnK-UUU-rps16_p2 CCGCACTTAAAAGCCGAGTA TTCAAAGAAGGCAGGGGTTT 50.0 45.0 58.3 57.5 842-868 bp 1.0 79.6
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA TTTCAAAGAAGGCAGGGGTT 50.0 45.0 58.3 57.5 843-869 bp 1.0 79.6
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA ATTTCAAAGAAGGCAGGGGT 50.0 45.0 58.3 57.3 844-870 bp 1.0 77.7
trnK-UUU-rps16_p5 CGCACTTAAAAGCCGAGTAC TCAAAGAAGGCAGGGGTTTT 50.0 45.0 57.2 57.5 840-866 bp 1.0 75.6