Hotspot

rps3

LSC · 83717-84373 · 657 bp

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Pi 0.00609

MarkerSeek score 62.30

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 4
pi: 0.00609
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	GGTTGGCCTTTTTATTGTTCCA	AGGTAGGCGAGTAGAGCTTT	40.9	50.0	57.7	57.9	811 bp	1.0	60.5
rps3_p2	GGTTGGCCTTTTTATTGTTCCA	AGGCGAGTAGAGCTTTTGTC	40.9	50.0	57.7	57.6	807 bp	1.0	58.7
rps3_p3	GGTTGGCCTTTTTATTGTTCCA	GGCGAGTAGAGCTTTTGTCT	40.9	50.0	57.7	57.6	806 bp	1.0	58.7
rps3_p4	GGTTGGCCTTTTTATTGTTCCA	CCCCTTAGGTAGGCGAGTAG	40.9	60.0	57.7	58.4	817 bp	1.0	58.6
rps3_p5	AGGTTGGCCTTTTTATTGTTCC	AGGTAGGCGAGTAGAGCTTT	40.9	50.0	57.4	57.9	812 bp	1.0	58.3