Hotspot

rps16

LSC · 5304-6454 · 1151 bp

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Pi 0.00349

MarkerSeek score 61.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1151
variable_sites: 4
pi: 0.00349
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TTGTGTTGGATTGGCGCTAT	TCCATAATTGAATCAACCGCA	45.0	38.1	58.2	55.9	1297-1302 bp	1.0	54.7
rps16_p2	TTGTGTTGGATTGGCGCTAT	GTTCTGACTGAACCAATGACT	45.0	42.9	58.2	55.8	1327-1332 bp	1.0	53.8
rps16_p3	TTGTGTTGGATTGGCGCTAT	AATTGAATCAACCGCATAGC	45.0	40.0	58.2	54.7	1292-1297 bp	1.0	53.0
rps16_p4	TTTGTGTTGGATTGGCGCTA	TCCATAATTGAATCAACCGCA	45.0	38.1	58.4	55.9	1298-1303 bp	1.0	52.9
rps16_p5	TTTTTGTGTTGGATTGGCGC	TCCATAATTGAATCAACCGCA	45.0	38.1	58.4	55.9	1300-1305 bp	1.0	52.6