Hotspot

psbH

LSC · 75485-75706 · 222 bp

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Pi 0.0045

MarkerSeek score 40.30

Primer available yes

Label psbH

Feature Metadata

label_name: psbH
feature_type: gene
region: LSC
length_bp: 222
variable_sites: 1
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH_p1	GTTCCGAGATGTTTTTGCCG	ATGCGGCTTTTTGCTTTTGT	50.0	40.0	58.0	58.0	1538 bp	1.0	79.8
psbH_p2	GTTCCGAGATGTTTTTGCCG	ATCCGACGAGGTAGAACCAT	50.0	50.0	58.0	57.9	1136 bp	1.0	79.8
psbH_p3	GTTCCGAGATGTTTTTGCCG	TGAATCGCTTGAACCCTCAG	50.0	50.0	58.0	57.9	1483 bp	1.0	79.5
psbH_p4	GTTCCGAGATGTTTTTGCCG	AATCGCTTGAACCCTCAGAC	50.0	50.0	58.0	57.9	1481 bp	1.0	79.4
psbH_p5	ACATCTGGCATGGGTCTAGA	ATGCGGCTTTTTGCTTTTGT	50.0	40.0	57.8	58.0	1563 bp	1.0	78.7