Hotspot

atpF

LSC · 11962-13371 · 1410 bp

Back to Celosia

Pi 0.00664

MarkerSeek score 66.30

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1410
variable_sites: 9
pi: 0.00664
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTTCGTCTGCTCGAATGGTT	AGCAAAGAAGTGAAATAATACAACGA	45.0	30.8	57.8	57.9	1507-1560 bp	1.0	43.3
atpF_p2	TCGTCTGCTCGAATGGTTAC	AGCAAAGAAGTGAAATAATACAACGA	50.0	30.8	57.7	57.9	1505-1558 bp	1.0	43.3
atpF_p3	GTCTGCTCGAATGGTTACCA	AGCAAAGAAGTGAAATAATACAACGA	50.0	30.8	57.6	57.9	1503-1556 bp	1.0	43.3
atpF_p4	TTTCGTCTGCTCGAATGGTT	GCAAAGAAGTGAAATAATACAACGA	45.0	32.0	57.8	56.7	1506-1559 bp	1.0	43.3
atpF_p5	ATTTCGTCTGCTCGAATGGT	AGCAAAGAAGTGAAATAATACAACGA	45.0	30.8	57.6	57.9	1508-1561 bp	1.0	43.3