Hotspot

psbT-psbN

LSC · 75185-75243 · 59 bp

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Pi 0.0847

MarkerSeek score 85.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 5
pi: 0.0847
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	CGAGAACCTCCTAAAGTTCCA	TTCGAGGAACACGGAGACTA	47.6	50.0	57.0	57.8	125 bp	1.0	66.0
psbT-psbN_p2	CGAGAACCTCCTAAAGTTCCA	TCGAGGAACACGGAGACTAG	47.6	55.0	57.0	58.3	124 bp	1.0	65.5
psbT-psbN_p3	CCTCCTAAAGTTCCAAGTCCA	TTCGAGGAACACGGAGACTA	47.6	50.0	56.9	57.8	119 bp	1.0	65.3
psbT-psbN_p4	ACCTCCTAAAGTTCCAAGTCC	TTCGAGGAACACGGAGACTA	47.6	50.0	56.9	57.8	120 bp	1.0	65.2
psbT-psbN_p5	CCTCCTAAAGTTCCAAGTCCA	TCGAGGAACACGGAGACTAG	47.6	55.0	56.9	58.3	118 bp	1.0	64.7