Hotspot

rps18-rpl20

LSC · 69187-69437 · 251 bp

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Pi 0.02

MarkerSeek score 64.40

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 251
variable_sites: 5
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	TTGAAAGAACCGAGTCGACT	GGAAAGAAGAATACCTCGAAACG	45.0	43.5	56.8	57.5	336 bp	1.0	44.4
rps18-rpl20_p2	CGAGTCGACTACTAGAACTGC	GGAAAGAAGAATACCTCGAAACG	52.4	43.5	57.7	57.5	326 bp	1.0	43.4
rps18-rpl20_p3	TTTGAAAGAACCGAGTCGACT	GGAAAGAAGAATACCTCGAAACG	42.9	43.5	57.6	57.5	337 bp	1.0	42.2
rps18-rpl20_p4	ACCGAGTCGACTACTAGAACT	GGAAAGAAGAATACCTCGAAACG	47.6	43.5	57.4	57.5	328 bp	1.0	41.5
rps18-rpl20_p5	TGAAAGAACCGAGTCGACTAC	GGAAAGAAGAATACCTCGAAACG	47.6	43.5	57.5	57.5	335 bp	1.0	41.4