Hotspot

psaJ-rpl33

LSC · 67928-68392 · 465 bp

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Pi 0.0304
MarkerSeek score 74.40
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
465
variable_sites
14
pi
0.0304
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTTTCCCAGATGCGTTGACA TCTGGAAACGCCCTTTGATC 45.0 50.0 58.0 58.2 591-596 bp 1.0 80.9
psaJ-rpl33_p2 ATGCGTTGACATTCCCCTTT TCTGGAAACGCCCTTTGATC 45.0 50.0 58.1 58.2 582-587 bp 1.0 80.6
psaJ-rpl33_p3 ACATATCTTTCGGTGGCACC TCTGGAAACGCCCTTTGATC 50.0 50.0 58.0 58.2 670-675 bp 1.0 80.5
psaJ-rpl33_p4 TTCCCAGATGCGTTGACATT TCTGGAAACGCCCTTTGATC 45.0 50.0 57.8 58.2 589-594 bp 1.0 79.5
psaJ-rpl33_p5 TTTCCCAGATGCGTTGACAT TCTGGAAACGCCCTTTGATC 45.0 50.0 57.8 58.2 590-595 bp 1.0 79.5