Hotspot

trnT-GGU-psbD

LSC · 32446-33741 · 1296 bp

Back to Celosia

Pi 0.017

MarkerSeek score 59.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1296
variable_sites: 22
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TTTACCAACGGCTATAGTCA	55.0	40.0	58.1	53.8	1375-1390 bp	1.0	47.4
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TTTACCAACGGCTATAGTCA	50.0	40.0	58.1	53.8	1378-1393 bp	1.0	47.2
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TTTACCAACGGCTATAGTCA	50.0	40.0	58.3	53.8	1352-1367 bp	1.0	45.7
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	TTTACCAACGGCTATAGTCA	50.0	40.0	57.7	53.8	1390-1405 bp	1.0	45.6
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	TTACCAACGGCTATAGTCAT	55.0	40.0	58.1	53.6	1374-1389 bp	1.0	45.4