Hotspot

petN-psbM

LSC · 29064-29889 · 826 bp

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Pi 0.00605

MarkerSeek score 62.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 826
variable_sites: 5
pi: 0.00605
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATTGTAAGTCTCGCTTGGGC	AAAAACGGTCAGCCAAAACG	50.0	45.0	58.3	58.1	935-943 bp	1.0	77.7
petN-psbM_p2	ATTGTAAGTCTCGCTTGGGC	ACGGTCAGCCAAAACGATTA	50.0	45.0	58.3	57.8	931-939 bp	1.0	77.0
petN-psbM_p3	TAGTATGGGGGAGAAGTGGG	AAAAACGGTCAGCCAAAACG	55.0	45.0	57.5	58.1	877-885 bp	1.0	75.9
petN-psbM_p4	TAGTATGGGGGAGAAGTGGG	ACGGTCAGCCAAAACGATTA	55.0	45.0	57.5	57.8	873-881 bp	1.0	75.2
petN-psbM_p5	ATTGTAAGTCTCGCTTGGGC	AACGGTCAGCCAAAACGATT	50.0	45.0	58.3	58.7	932-940 bp	1.0	72.9