Hotspot

trnS-GCU-trnG-UCC

LSC · 8467-9312 · 846 bp

Back to Celosia

Pi 0.00361

MarkerSeek score 61.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 3
pi: 0.00361
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ACGGAAAGAGAGGGATTCGA	GTATTCAAACCAAACCCGCG	50.0	50.0	58.2	58.0	1012-1018 bp	1.0	79.9
trnS-GCU-trnG-UCC_p2	CGAATGACTCGTACAACGGA	GTATTCAAACCAAACCCGCG	50.0	50.0	57.7	58.0	982-988 bp	1.0	79.1
trnS-GCU-trnG-UCC_p3	TGGAAACGGAAAGAGAGGGA	GTATTCAAACCAAACCCGCG	50.0	50.0	58.3	58.0	1017-1023 bp	1.0	78.9
trnS-GCU-trnG-UCC_p4	CTTTCGTCCACTCAGCCATC	GTATTCAAACCAAACCCGCG	55.0	50.0	58.6	58.0	948-954 bp	1.0	76.1
trnS-GCU-trnG-UCC_p5	AACGGAAAGAGAGGGATTCG	GTATTCAAACCAAACCCGCG	50.0	50.0	57.3	58.0	1013-1019 bp	1.0	75.7