Hotspot

psbI-trnS-GCU

LSC · 8150-8378 · 229 bp

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Pi 0.0136

MarkerSeek score 64.10

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 3
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATCCTGGGCGCGAAGAATA	GTCATTCGTACCGAGGGTTC	50.0	55.0	59.5	58.1	278-287 bp	1.0	70.5
psbI-trnS-GCU_p2	ATCCTGGGCGCGAAGAATAA	GTCATTCGTACCGAGGGTTC	50.0	55.0	59.5	58.1	277-286 bp	1.0	70.5
psbI-trnS-GCU_p3	TAATCCTGGGCGCGAAGAAT	GTCATTCGTACCGAGGGTTC	50.0	55.0	59.5	58.1	279-288 bp	1.0	70.5
psbI-trnS-GCU_p4	ATCCTGGGCGCGAAGAATA	GTCATTCGTACCGAGGGTTC	52.6	55.0	58.9	58.1	277-286 bp	1.0	67.8
psbI-trnS-GCU_p5	ATCCTGGGCGCGAAGAATAA	AGTCATTCGTACCGAGGGTT	50.0	50.0	59.5	58.4	278-287 bp	1.0	67.6