Hotspot

ycf4-cemA

LSC · 60932-61489 · 558 bp

Back to Celastrus

Pi 0.0239

MarkerSeek score 70.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 558
variable_sites: 50
pi: 0.0239
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ATTTCTTGCGTGTCCCCATT	ATAGAAGGGGAGTGAACGCT	45.0	50.0	58.1	58.1	624-638 bp	1.0	88.4
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	ATAGAAGGGGAGTGAACGCT	45.0	50.0	58.1	58.1	631-645 bp	1.0	88.2
ycf4-cemA_p3	CTATTTCTTGCGTGTCCCCA	ATAGAAGGGGAGTGAACGCT	50.0	50.0	57.9	58.1	626-640 bp	1.0	88.2
ycf4-cemA_p4	CGTGTCCCCATTGAAGTCTT	ATAGAAGGGGAGTGAACGCT	50.0	50.0	57.8	58.1	616-630 bp	1.0	87.7
ycf4-cemA_p5	ATTTCTTGCGTGTCCCCATT	TAGAAGGGGAGTGAACGCTT	45.0	50.0	58.1	58.4	623-637 bp	1.0	86.7