Hotspot

ndhC-trnV-UAC

LSC · 51725-52191 · 467 bp

Back to Celastrus

Pi 0.0158

MarkerSeek score 58.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 467
variable_sites: 27
pi: 0.0158
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCCAGAAAAGATCGTATTCG	GCATGTCGGGTCTTTGAAAC	47.6	50.0	57.1	57.7	544-599 bp	1.0	70.6
ndhC-trnV-UAC_p2	ACTCCGGAAATTAGAAATGCCA	GCATGTCGGGTCTTTGAAAC	40.9	50.0	58.0	57.7	598-653 bp	1.0	68.8
ndhC-trnV-UAC_p3	GCCCAGAAAAGATCGTATTCG	AGAAGGTCTACGGTTCGAGT	47.6	50.0	57.1	57.8	480-535 bp	0.916667	68.2
ndhC-trnV-UAC_p4	ACTCCGGAAATTAGAAATGCCA	AGAAGGTCTACGGTTCGAGT	40.9	50.0	58.0	57.8	534-589 bp	0.916667	66.4
ndhC-trnV-UAC_p5	GAAATGCCCAGAAAAGATCGT	AGAAGGTCTACGGTTCGAGT	42.9	50.0	56.9	57.8	485-540 bp	0.916667	66.0